Abstract:

Abstract:Objective A comparative study of hyperlipidemia models induced by fat emulsion in SD rats and KM mice was conducted in order to provide reference for the study of the pathogenesis of hyperlipidemia and the basis for the selection of animal disease models for the development of lipid-lowering drugs. Method The hyperlipidemia model was induced by gavage of fatty emulsion for 2 weeks and 4 weeks, and the four blood lipids ( TG, TC, LDLC, HDL-C) , liver fat ( TG, TC) and liver tissue lesions were detected. Result Compared with the normal group, the fat emulsion of KM mice was intragastrically administered for 2 weeks, and there was no obvious change in blood lipids. After 4 weeks of intragastric administration, the liver tissues showed mild pathological damage, but blood lipids and liver lipids remained unchanged. SD rat fat emulsion was given to the stomach for 2 weeks, serum TC and LDL-C were significantly increased (P<0. 001, P<0. 01) , HDL-C was significantly reduced (P<0. 001) , and TG had no significant change; fat emulsion was given to the stomach 4 In week, rat serum TG, TC, LDL-C were significantly increased (P<0. 001, P<0. 01) , HDL-C was significantly reduced ( P < 0. 001) , and the liver was obviously enlarged, white in color, and liver tissue lesions. The degree is heavier, with obvious fat vacuoles. Conclusion The hyperlipidemia model of SD rats induced by fat emulsion gavage for 4 weeks was better.

Key words:

Key words:hyperlipidemia model, fat emulsion, comparative study, blood lipids, liver lipids

摘要：

摘要:目的 通过脂肪乳诱导 SD 大鼠与昆明种小鼠高脂血症模型的对比研究,以期为高脂血症发病机制的研究提供参考,并为降脂药物的研发提供动物疾病模型选择的依据。 方法 通过脂肪乳分别对昆明种小鼠及 SD 大鼠灌胃 2 周和 4 周诱导高脂血症模型,检测动物血脂 4 项( TG、TC、LDL-C、HDL-C) 、肝脂( TG、TC) 及肝组织病变程度。结果 与对照组比较,昆明种小鼠脂肪乳灌胃 2 周,血脂无明显变化;灌胃 4 周,可见肝组织出现轻度病理损伤,但血脂和肝脂仍无明显变化。 SD 大鼠脂肪乳灌胃 2 周,血清 TC、LDL-C 明显升高( P<0. 001、P<0. 01) ,HDL-C 明显降低( P<0. 001) ,TG 无明显变化;脂肪乳灌胃 4 周,大鼠血清 TG、TC、LDL-C 明显升高( P< 0. 001、P< 0. 01) ,HDL-C 明显降低( P< 0. 001) ,并可见肝脏明显肿大,颜色发白,肝组织病变程度较重,有明显的脂肪空泡出现。 结论 脂肪乳灌胃 4 周诱导 SD 大鼠高脂血症模型较佳。

关键词:

关键词: 高脂血症模型, 脂肪乳, 对比研究, 血脂, 肝脂